venipuncture; discard 1st mL of blood by collecting a discard tube prior to
collecting the blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube should also be collected
prior to collection of the blue top to ensure sufficient sample volume. Drawing
a discard tube will displace the air from the blood collection set tubing to
ensure proper blood draw volume.
For patients on heparin
therapy, the whole blood sample must be centrifuged within 1 hour of collection.
Specimen Preparation: Acceptable up to 4
draw, at ROOM TEMPERATURE (20 +/- 5°C).
If delivery time
is to be greater than 4 hours from time of draw,
specimen for 15 minutes at 2000-2500g. Separate the
plasma from the cells and freeze at -200C.
Plasma 4 hrs at 20 +/-
on heparin therapy -plasma is stable for 2 hrs at 20 +/- 5°C when collected in 3.2% sodium
citrate (blue top). Frozen plasma 2 Weeks at -20°C. Whole
blood is stable for 4 hours at room temperature.
Causes for Rejection:
severely hemolyzed, greater than 4 hours old,
wrong tube (3.8%
sodium citrate), high hematocrit (> 55%), sample identification
error, processed and/or stored improperly.