Endocervix and ectocervix should
be separately sampled.
Entire ectocervix sampled by
rotating a wooden or plastic Ayre spatula with pressure.
Endocervix sampled with a
pointed Ayre spatula rotating it 1-2 full turns (use of Cytobrush enhances cell
Apply the material rapidly to a
glass slide labeled in pencil with the patient's name and ID #; spread evenly.
Fix the slide immediately by
immersing it in 95% ethyl alcohol x 15 minutes, or spraying with aerosol or pump
In older women, a posterior
vaginal pool or cervical canal aspiration should be taken to detect endometrial
Collection of SurePath™ Thin Layer Cervical Sample
Insert the Rover's Cervex-Brush®
into the endo-cervical canal.
Apply gentle pressure until the
bristles form against the cervix.
Maintaining gentle pressure,
hold the stem between the thumb and forefinger and rotate the brush five times
in a clockwise direction.
Remove the Rover's Cervex-Brush®
from the patient.
Placing your thumb against the
back of the brush, disconnect the entire brush from the stem, dropping it into
the CytoRich® preservative vial.
Place the cap on the vial and
Label the vial with patient's
full name and ID number and/or physician name and date.
Complete the Cytopathology
requisition form with the required information.
Put the vial and the matching
requisition into a specimen bag and send to the Cytopathology lab.
Storage of Non-Gynecological Cytology Specimens
Body cavity fluids (pleural, abdominal,
pericardial). Store specimens in refrigerator (4°C - 8°C) - up to 48
hours. (During the regular laboratory hours, specimens should be sent
directly to Cytology Laboratory immediately).
Sputum, bronchial washings, bronchioalveolar lavage
(BAL). Store specimens in refrigerator (4°C - 8°C) - up to 48 hours.
(During the regular laboratory hours, specimens should be sent directly to
Cytology Laboratory immediately).
Spinal fluids, ventricular fluids, urine. Fix
specimens (fluids) with equal amount of 50% Alcohol and store in refrigerator
(4°C - 8°C). (During the regular laboratory hours, no fixative should be
added to specimens. All specimens should be sent directly to Cytology
Specimens collected during the weekends, holidays,
and after the regular hours can be delivered and stored in the refrigerator
outside the specimen receiving area of Cytopathology.
Reviewed 6/22/12 by Robert Stewart