Bhupendra S. Kaphalia, Ph.D.
Associate Professor
Affiliations: Department of Pathology
Phone: (409) 772-4995
Fax: (409) 747-1763
Email: bkaphali@utmb.edu
Education
1971 - 1973 B.S., Chemistry, Zoology, Botany - Agra University, Agra, India
1973 - 1975 M.S, Chemistry - Kumaun University, Nainital, India
1980 - 1984 Ph.D., Chemistry/Toxicology - Kumaun University, Nainital,
India
Research Interests
The primary focus of my research is to understand the molecular mechanisms of toxic action of the xenobiotic alcohols, which involves studies related to metabolomics, toxicokinetics, and biochemical toxicity.
One of the potential mechanism of retention and toxicity of xenobiotics and/or their metabolites is associated with their conjugation with fatty acids and other membrane lipids via non-oxidative metabolism catalyzed by fatty acid ethyl ester synthase. These metabolites have been implicated in the etiology of chronic diseases of substance abuse and environmental exposure. I use state of the art instrumentation such as HPLC, nuclear magnetic resonance and mass spectrometry, and develop analytical methods. These studies are important for elucidating the toxic metabolic pathways of the xenobiotics and/or their metabolites.
Purification and characterization of the enzymes involved in the metabolism of xenobiotics and/or their metabolites are also the part of ongoing research on understanding the mechanism(s) of toxic action of the parent compound. My current research is directed to delineate the molecular mechanism and development of preventive measures of alcoholic pancreatitis and hepatitis.
Biographical Information
1998 - present Assistant Professor, Department of Pathology
University of Texas Medical Branch. Galveston, TX
1988 - 1998 Instructor, Department of Pathology
University of Texas Medical Branch. Galveston, TX
1985 - 1988 Postdoctoral Fellow, Department of Pathology
University of Texas Medical Branch. Galveston, TX
1978 - 1985 Scientific Assistant
Industrial Toxicology Center. Lucknow, India.
1977 - 1978 Junior Research Fellow
Council of Scientific and Industrial Research. New Delhi, India.
1976 - 1977 Junior Research Fellow, World Health Organization
Industrial Toxicology Research Center. Lucknow, India.
Selected Publications
Zeng FY, Kaphalia BS, Ansari GAS, Weigel PH: Fatty acylation of the rat asialoglycoprotein receptor: The three subunits from active receptors contain covalently bound palmitate and stearate. J Biol Chem 270:21382-21387, 1995
Kaphalia BS, Fritz RR, Ansari GAS: Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (pl 6.1). Chem Res Toxicol 10:211-218, 1997
Kaphalia BS, Green SM, Ansari GAS: Fatty acid ethyl and methyl ester synthases, and fatty acid anilide synthase in HepG2 and AR42J cells: Interrelationships and inhibition by tri-O-tolyl phosphate. Toxicol Appl Pharmacol 159:134-141, 1999
Kaphalia BS and Ansari GAS: Purification and characterization of rat hepatic microsomal low molecular weight ethyl ester synthase and their relationship to carboxylesterases. J Biochem Mol Toxicol 15: 165-171, 2001.
Kaphalia BS, Khan MF, Green SM, Mericle KA, and Ansari GAS Xenobiotic conjugates of fatty acids. Pharmacology and Therapeutics in the New Millennium. pp 698-706, 2001.
Kaphalia BS and Ansari GAS. Fatty acid ethyl esters and ethanol-induced pancreatitis. Cell Mol Biol 47: OL173-OL179, 2001.
Kaphalia BS and Ansari GAS. Purification and characterization of rat pancreatic fatty acid ethyl ester synthase and its structural and functional relationship to pancreatic cholesterol esterase. J Biochem Mol Toxicol 17; 338-345, 2003.
Kaphalia BS, Mericle KA, and Ansari GAS: Mechanism of Differential Inhibition of Hepatic and Pancreatic Fatty Acid Ethyl Ester Synthase by Inhibitors of Serine-Esterases: In Vitro and Cell Culture Studies. Toxicol Appl Pharmacol 200; 7-15. 2004.
Kaphalia BS, Cai Ping, Khan MF, Okorodudu, AO and Ansari GAS: Fatty acid ethyl esters in alcoholics; markers of alcohol abuse and alcoholism. Alcohol 34, 151-158, 2004.