asthma pathogenesis
 

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General Information

Epithelial Signaling Program Project (PO1)

Individual Projects:


Other Asthma Activities


 

Viral Tissue Culture and Immunoassay Core
(PI:Garofalo, R)



Contact Information:

Roberto P. Garofalo, MD
Professor in Pediatrics
2.36 Childrens Hospital
Department of Pediatrics UTMB
301 University Blvd.
Galveston, TX 77555-1060
Email:   rpgarofa@utmb.edu

Dr. Garofalos's bio...

Roberto Garofalo

airway inflammation

Project Summary:

As Co-PI, Dr. Garofalo brings particular expertise to the overall project. In this capacity, Dr. Garofalo is responsible for the overall direction of the PO1 and oversight of the animal and human subjects research being conducted in all four of its projects.

Dr. Garofalo also directs Core B - the Viral, Tissue Culture and Immunoassay Core (VTCIC) facility, which supports all four projects included in this Program Project. In this capacity, Core B serves as a centralized resource for cell culture maintenance, cellular and viral purification, immunossay performance, and mouse lung pathophysiology studies. Support of the VTCIC facility is justified based on the Programís critical need for a number of biological reagents, methodological procedures immunological assays, and studies on airway function in mice which are common to all four projects. Centralized acquisition of reagents and performance of tests will minimize costs and result in optimal standardization of assays and experimental procedures. The facility will take advantage of resources currently available to all P01 investigators at UTMB including the Child Health Research Center laboratory, a 7,500 sq. ft., state-of-art facility where Core B will be located. Its central location on the UTMB campus will facilitate communication among the investigators of this Program Project, resulting in more efficient and collaborative research.


Specific objectives of the Core are to provide the infrastructure necessary to prepare reagents and perform the following assays:

  • Maintain a tissue bank of human cell lines (mostly of epithelial origin);
  • Prepare pools of sucrose-purified respiratory syncytial virus (RSV);
  • Perform immunoassays for human and mouse cytokines and chemokines; and 5. Assist investigators in performing lung physiology studies using the Buxco and flexiVent platforms.
The results obtained from these studies will help elucidate an important and novel molecular pathway by which respiratory viruses induce lung inflammation, and should lay the foundation for future studies to develop novel therapeutic strategies against lower respiratory tract infections and virus-triggered precipitation of asthma attacks.




Recent Publications:


  1. Garofalo, R.P. & Haeberle, H. (2000). Epithelial regulation of innate immunity to RSV. Am. J. Respir. Cell Biol, 23:581-585
  2. Garofalo, R.P., Patti, J., Hintz, K.A., Hill, V., Ogra, P.L., & Welliver, R.C. (2001). Macrophage Inflammatory Protein-1alpha (Not T Helper Type 2 Cytokines) Is Associated with Severe Forms of Respiratory Syncytial Virus Bronchiolitis. J Infect Dis, 184: 393-399.
  3. Kolli D, Bao X, Liu T, Hong C, Garofalo R.P., and Casola, A. (2011). Human metapneumovirus glycoprotein G modulates Toll-like receptor-4 signaling in monocyte-derived dendritic cells. J Immunol, 2011;187:47-54. PMCID: PMC3119724
  4. Dey N, Liu T, Garofalo RP and Casola A. (2011). TAK1 regulates NF-kB and AP-1 activation in airway epithelial cells following RSV infection. Virology, 418:93-101.
  5. Castro, S.M., Guerrero-Plata, A., Suarez-Real, G., Abegboyega, P.A., Colasurdo, G.N., Khan, A.M., Garofalo, R.P. & Casola, A. (2006). Antioxidant treatment ameliorates RSV-induced disease and pulmonary inflammation. Am J Resp and Criti Care Med, 174:1361-69.
  6. Hosakote, Y.M., Jantzi, P., Esham, D., Kurosky, A., Casola, A. and Garofalo, R.P. (2011). Viral-mediated inhibition of antioxidant enzymes contributes to the pathogenesis of severe RSV bronchiolitis. Am J Resp Critic Care Med,183:1550-60. PMCID:PMC3137144
  7. Hosakote, Y.M., Komaravelli, N., Mautemps, N., Liu, T., Garofalo, R.P., and Casola, A. (2012). Antioxidant mimetics modulate oxidative stress and cellular signaling in airway epithelial cells infected with respiratory syncytial virus. Am J Physiol Lung Cell Mol Physiol, 303:L991-1000. PMCID:PMC3532525
  8. Bao X, Kolli D, Liu T, Garofalo R.P and Casola A. (2013). Human Metapneumovirus Glycoprotein G Disrupts Mitochondrial Signaling in Airway Epithelial Cells. PLoS One, 8:e62568
  9. Jamaluddin, M., Wiktorowicz, J.E., Soman, K.V., Boldogh, I., Forbus, J.D., Spratt, H., Garofalo, R.P., & Brasier, A.R. (2010). Role of Peroxiredoxin 1 and Peroxiredoxin 4 in protection of respiratory syncytial virus-induced cysteinyl oxidation of nuclear cytoskeletal proteins. J Virol, 84: 9533-9545. PMCID: PMC2937607


 

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