UTMB
  UTMB - Biomolecular Resource FacilityHomeAboutCoresResourcesLinks  
Protein Chemistry
Online Sample Order Form Under Construction Online Sample Order Form
     
     
     
     
     
 
Galveston, Texas
Biomolecular Resource Facility
301 University Boulevard
Galveston, Texas 77555-0144
 
   
   
   

Edman Sequencing Chemistry

Taken from : Sigma-Aldrich Co. The figure below shows a simplified scheme of the Edman chemistry. Coupling of N-terminal amino groups of protein (1) with phenylisothiocyanate (PITC, Edman reagent) (2) forming an open chain thiourea derivative (3), the N-terminal amino acid is split off as anilinothiazolinone (ATZ) derivative (5) with trifluoroacetic acid. This unstable ATZ-derivative is transferred into a more stable phenylthiohydantoin (PTH) derivative (6), which is separated by reversed phase HPLC and identified by comparison with a standard.

 

Edman Sequencing Chemistry

 

Return to Protein Chemistry Core

 
 

Home | About | Cores | Resources | Links
UTMB
| Search | Directory | Toolbox | News | Jobs | Contact | Sitemap 
UT System | Reports to the State | Compact With Texans | Statewide Search
 
This site published by John E. Wiktorowicz, Ph.D. for the Biomolecular Resource Facility. 
Copyright ©  2004-05  The University of Texas Medical Branch. Please review our privacy policy and Internet guidelines.

 
NHLBI Proteomics Home E-mail