About Us

The UTMB NIAID Clinical Proteomics Center for Infectious Disease and Biodefense offers established proteomic expertise to support the discovery, qualification and verification of clinical protein biomarkers for diseases caused by NIAID Category A-C pathogens and emerging and re-emerging infectious disease pathogens. This Center draws on existing resources in the Sealy Center for Molecular Medicine, the UTMB Proteomics Center, the UTMB Bioinformatics Program, and the Center for Biodefense and Emerging Infectious Diseases (CBEID). A clinical proteomics pipeline draws in unique intellectual expertise in NIAID Category A-C pathogens and an international network of contacts and collaborators, including, for example, the NIAID Region V Centers of Excellence for Biodefense and Emerging Infectious Diseases (WRCE) and the WHO Reference Library on Arbovirus located at UTMB.

Overview

The UTMB CPC Center is divided into separate Technical Teams to maximally align with the major scientific and technical objectives of this contract. The major pathway for information and sample flow is indicated by the green arrows. Clinical studies will be developed via the Clinical Proteomics Pipeline and evaluated by our Scientific Working Group (SWG).

Administrative Team.

The major activities of the Administative Team are to:

  • Coordinate reviews of biomarker project applications developed in the Clinical Proteomics Pipeline with the Scientific Working Group (SWG);
  • Ensure that projects meet appropriate regulatory requirements, and to monitor time lines and milestones;
  • Act as the primary resource for communicating CPC activities to outside scientists; actively maintain a web portal for communication with the scientific community and data exchange with the Administrative Resource and Data Centers for the Biodefense Proteomics Research Centers.

Sample Repository/Data Warehouse Team.

The Sample Repository/Data Warehouse Team is responsible for the data archival and tracking of samples submitted to the UTMB CPC. The aims of the Sample Repository/Data Warehouse Team are to:

  • Establish a Sample Repository, a site for secure and compliant storage of biological samples and information submitted for biomarker discovery or verification;
  • Design, test, optimize and deploy stable and secure relational databases capturing clinical and proteomics datasets for bioinformatics discovery of biomarker panels;
  • Submit candidate biomarkers, project data and other related information to public repositories and other collaborating sites, including NIAID Bioinformatics Resources Center program.

Proteomics Discovery Team.

The Discovery Team is co-directed by John Wiktorowicz, PhD and Alexander Kurosky, PhD, nationally recognized investigators in proteomics and technology development. The aims of the Proteomics Discovery Team are to:

  • Conduct unbiased differential proteomics analysis using multidimensional fractionation (LC, HPLC, 2DE) followed by MS/MS-based identification techniques;
  • Perform primary image analysis of 2DE and construct reference gels of replicate runs;
  • Determine protein identifications by quantitative analysis of stable isotopic labeling in MS;
  • Track workflow and update results in the proteomics LIMS.

The Bioinformatics Team.

The Bioinformatics Team is directed by Bruce Luxon, PhD, Director of the UTMB Bioinformatics Program. He will be supported by statisticians and programmers to identify groups (panels) of disease-informative biomarkers and construct models that associate these biomarkers with clinical outcome. The aims of the Bioinformatics Team are to:

  • Conduct supervised (machine learning) analysis to generate predictive models of candidate biomarkers;
  • Compare and evaluate predictive models for sensitivity and specificity;
  • Prioritize panels of biomarkers for further qualification and verification.

Qualification and Verification Team.

The Qualification and Verification Team is directed by Yingxin Zhao. The team apply independent methods to confirm protein identification and to allow for larger verification studies of candidate biomarkers. The aims of the Qualification and Verification Team are to:

  • Qualify protein identifications prioritized by the Bioinformatics Team;
  • Develop verification platforms for predictive model testing using reverse phase microarrays, multiplex ELISA or multiple reaction monitoring.

Galveston, TX

UTMB

UTMB

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GNL

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Contact

Allan Brasier, MD
Email: arbrasie@utmb.edu