Biomedical Resource Facility
Mass Spectrometry Core
provides a) MALDI – TOF/TOF MS/MS analysis; b) electrospray ionization MS/MS analysis, including liquid chromatography (LC/MS/MS); c) protein ID analysis; d) post-translational modification identifications, and e) stable isotope methods (e.g. iTRAQ and 18 O).
Proteomics Bioinformatics Core
provides a) nonlinear 2D gel analysis; b) Genologics LIMS for data/sample management and access, and c) database searching.
Separations Technology Core
provides a) HPLC analysis; b) 1D and 2D gel electrophoreses; c) fluorescence gel staining and imaging, and d) robotic gel spot picking and processing for MS analysis.
Department of Microbiology and Immunology
facility is equipped with one high-speed cell sorter as well as three analyzers suitable for various tasks and levels of user friendliness. The BD LSRFortessa and FacsCanto are advanced analyzers that can detect 18 and 6 colors, respectively. The Fortessa offers automatic sample loading with a 96 well plate reader that allows for walk-away use. The Canto has a carousel loader with similar convenience features. The third analyzer, Accuri C6, is capable of detecting 4 colors, and is particularly user friendly. All analyzers are available to independent users with 24/7 access.
Tissue Culture Core Facility
is a division of the Department of Microbiology and Immunology. The core supports UTMB investigators in a time- and cost-efficient manner. The facility provides products for tissue culture, molecular biology, and immunology research from multiple vendors. Other services include heat in-activation of serum, mycoplasma detection and eradication, tissue culturing, and cryogenic preservation and repository of cells.
Sealy Center for Molecular Medicine
Molecular Genomics Core
provides services, technical support and advice to UTMB investigators in the area of genomic analysis. The MG Core provides gene expression analysis through the use of Affymetrix gene chips and real time arrays, SNP analysis (both association-based studies and discrimination assays), real time quantitative RT-PCR, miRNA analysis, DNA sequence analysis and high resolution melt analysis.
Recombinant DNA Lab
provides UTMB investigators with a variety of essential services and technical support in the area of recombinant DNA techniques. Services include the production of plasmid DNA, transformation and screening of recombinant plasmids, bacterial expression, site directed mutagenesis, construction of transgenic and null gene plasmids and various PCR based technologies.