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Antiphospholipid Standardization Laboratory (APLS)

Silvia Pierangeli, PhD, Lab Director
2.108 Brackenridge Hall  |  Galveston TX 77555-0883

Phone: 409-747-2126  |  email: sspieran@utmb.edu
Lab: 409-772-0225  |  Fax: 409-772-0226

Available Tests

Updated 12/4/2012

IMMUNOLOGY

COAGULATION

MOLECULAR GENETICS

Anticardiolipin Ab by ELISA (IgG, IgM, IgA)

Anti-β2 Glycoprotein I by ELISA (IgG, IgM, IgA)

ANA Profile: (anti-dsDNA, anti-SSA, anti-SSB, anti-Histone, anti-Centromere, anti-Jo-1, anti-RNP, anti-Scl70, anti-Sm by ELISA)

Anti-Thyroglobulin Ab

Anti-TPO (ELISA)

Anti-CCP Ab (ELISA)

Protein C activity and antigenic

Protein S activity and antigenic

Anti-thrombin III antigenic

Factor V Leiden by PCR

Prothrombin 20120 mutation (PCR)

MTHFR by PCR

 

SPECIMEN COLLECTION REQUIREMENTS, STORAGE, & STABILITY

ANTICARDIOLIPIN AB (IgG, IgM, IgA); ANTI-β2 GLYCOPROTEIN I AB (IgG, IgM, IgA), ANA PROFILE, ANTI-CCP AB) 

Collect: One 4 mL serum separator tube.

Storage/Transport Temperature: 2 mL serum at 2-8°C. (Min: 0.6 mL)

Remarks: Separate serum from cells ASAP. Avoid repeated freeze/thaw cycles.

Unacceptable Conditions: Plasma. Severely lipemic, contaminated, or hemolyzed specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year

THYROGLOBULIN ANTIBODIES

Collect: One 6 mL serum separator tube. Also acceptable: green (sodium or lithium heparin).

Storage/Transport Temperature: 1 mL serum or plasma at 2-8°C. (Min: 0.8 mL)

Unacceptable Conditions: EDTA Plasma.

Stability (collection to initiation of testing): After separation from cells: Ambient: 8 hours; Refrigerated: 2 days; Frozen: 6 months

TPO ANTIBODIES

Collect: One 4 mL serum separator tube. Also acceptable lavender (EDTA), pink (K2 EDTA), or green (sodium or lithium heparin).

Storage/Transport Temperature: 0.5 mL serum or plasma at 2-8°C. (Min: 0.3 mL

Unacceptable Conditions: Grossly hemolyzed or lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 8 hours; Refrigerated: 2 days; Frozen: 6 months and freeze.

Unacceptable Conditions: Specimens left to clot at 2-8°C.

PROTEIN S and PROTEIN C (Activity and Antigenic) and ANTI-THROMBIN III (Antigenic)

Collect: Two 5 mL (lt. blue sodium citrate). (Min: two 3 mL) Refer to the Hemostasis/Thrombosis Specimens section in the front of this User's Guide for specimen collection instructions.

Storage/Transport Temperature: 4 mL platelet-poor plasma, frozen. (Min: 1.5 mL) Submit specimen in an ARUP Standard Transport Tube.

Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. List medications.

Unacceptable Conditions: Serum. Non-frozen or hemolyzed specimens.

Stability (collection to initiation of testing): Ambient: 2 hours; Refrigerated: 4 hours; Frozen: 2 weeks

FACTOR V LEIDEN; PROTHROMBIN 20120 MUTATION (PCR), MTHFR (PCR)

Collect: One 3 mL lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Storage/Transport Temperature: 3 mL whole blood at 2-8°C. (Min: 1 mL)

Stability (collection to initiation of testing): Ambient: 3 days; Refrigerated: 1 week; Frozen: Unacceptable

Reviewed: 12/04/12 S. Pierangeli, PhD

 

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