Specimen Collection

Urine Specimen Collection

Random Urine Collection

First voided morning specimens should be collected in clean containers and transported to the laboratory within 30 minutes or stored according to the specific instructions

Timed Urine Collection

  • Containers (brown plastic bottles) for timed urine collections are provided by Sample Management, room 5.136 McCullough

  • Appropriate preservative is added to the container by the laboratory personnel when necessary. Refer to the alphabetical listing of tests for collection instructions for the required laboratory test.
  • Appropriate precautionary labels are affixed to the collection bottles containing hazardous chemicals. Read and follow the precautions.
  • Specimens requiring refrigeration may be placed in a basin filled with crushed ice if a refrigerator is not available.

To ensure accurate results, it is essential that an accurate collection of the timed output of urine is achieved. Follow these guidelines to collect a timed urine:

  1. At the beginning of the collection period, the bladder should be emptied.
  2. Discard this sample and note the time on the requisition form.
  3. Collect all urine passed from this point on.
  4. Specimens are placed in the timed urine bottle which may contain preservatives. Use caution when adding the urine to bottles containing acid preservatives. For safety purposes, instruct the patient to urinate in a clean, dry container, then transfer the urine to the collection bottle. The patient should not urinate into the collection bottle.
  5. If one of the samples is accidentally lost or removed within the collection period, the entire collection must be restarted.
  6. At the end of the collection period the bladder is emptied and the specimen is added to the collection.
  7. Note the ending time on the request form.
  8. If removing an aliquot of urine, the total volume for the timed collection must be noted on the request form.

Clean Catch, Mid-Stream Urine collection:

Females

  1. Remove appropriate garments for urination.
  2. Wash hands thoroughly.
  3. With one hand, separate the urinary opening and hold open until the process is complete.
  4. Using a saturated soapy sponge, wash from front to back three times using a different soap and pad each time.
  5. Wash with warm sterile water to remove soap residue.
  6. The patient should void a few milliliters into the toilet and then collect urine in a sterile container. (Do not stop the flow of urine through this process.)
  7. Secure the lid on the container.
  8. The nurse will transfer the specimen for culture to the gray-topped vacutainer tube through needle apparatus on urine cup. Refer to package insert for further information/instructions.

Males

  1. Remove appropriate garments for urination
  2. Wash hands thoroughly with soap and water.
  3. Cleanse the penis, retract the foreskin (if not circumcised), and wash three times with a different soap pad each time.
  4. Wash with warm sterile water to remove the soap residue.
  5. Keeping the foreskin retracted, allow a few milliliters of urine to pass and then collect urine in a sterile container. (Do not stop the flow of urine through this process.
  6. Secure the lid on the container.
  7. The nurse will transfer the specimen for culture to the gray-topped vacutainer tube through needle apparatus on urine cup. Refer to package insert for further information/instructions.