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SPECIMEN COLLECTION AND HANDLING
Pap Smears
[ Biopsy Tissue ] [ Blood Cultures ] [ Coagulation ] [ CSF ] [ Cytogenetics ] [ Cytopathology ] [ Fecal ] [ Pap Smears ] [ Throat Culture ] [ Urine Collection ] [ Viral ] [ Wound Sample ]
General
Considerations
Collection
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Endocervix and
ectocervix should be separately sampled.
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Entire ectocervix
sampled by rotating a wooden or plastic Ayre spatula with pressure.
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Endocervix sampled
with a pointed Ayre spatula rotating it 1-2 full turns (use of Cytobrush
enhances cell recovery).
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Apply the material
rapidly to a glass slide labeled in pencil with the patient’s name and ID #;
spread evenly.
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Fix the slide
immediately by immersing it in 95% ethyl alcohol x 15 minutes, or spraying
with aerosol or pump fixative.
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In older women, a
posterior vaginal pool or cervical canal aspiration should be taken to
detect endometrial lesions.
Collection of SurePath™ Thin Layer
Cervical Sample
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Insert the Rover's
Cervex-Brush® into the endo-cervical canal.
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Apply gentle
pressure until the bristles form against the cervix.
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Maintaining gentle
pressure, hold the stem between the thumb and forefinger and rotate the
brush five times in a clockwise direction.
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Remove the Rover's
Cervex-Brush® from the patient.
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Placing your thumb
against the back of the brush, disconnect the entire brush from the stem,
dropping it into the CytoRich® preservative vial.
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Place the cap on
the vial and tighten.
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Label the vial
with patient's full name and ID number and/or physician name and date.
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Complete the
Cytopathology requisition form with the required information.
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Put the vial and
the matching requisition into a specimen bag and send to the Cytopathology
lab.
Storage of Non-Gynecological
Cytology Specimens
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Body cavity fluids (pleural, abdominal, pericardial). Store
specimens in refrigerator (4°C - 8°C) - up to 48 hours. (During the regular
laboratory hours, specimens should be sent directly to Cytology Laboratory
immediately).
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Sputum, bronchial washings, bronchioalveolar lavage (BAL).
Store specimens in refrigerator (4°C - 8°C) - up to 48 hours. (During the
regular laboratory hours, specimens should be sent directly to Cytology
Laboratory immediately).
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Spinal fluids, ventricular fluids, urine. Fix specimens
(fluids) with equal amount of 50% Alcohol and store in refrigerator (4°C -
8°C). (During the regular laboratory hours, no fixative should be added to
specimens. All specimens should be sent directly to Cytology Laboratory
immediately).
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Specimens collected during the weekends, holidays, and after
the regular hours can be delivered and stored in the refrigerator outside
the specimen receiving area of Cytopathology.
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