Laboratory Test Directory

LYMPHOCYTE STIMULATION WITH PHA (082-7241)

Clinical Indication:
Patient with suspected Immunodeficiency

Test Mnemonic:
PHA

Methodology:
T Cell stimulation by 3H uptake

Performed:
Samples MUST be delivered to Pediatric Immunology/Allergy, 8:00 AM-Noon, Monday-Friday, Room C2.360, Research Building 006. PRIOR NOTIFICATION IS REQUIRED.

Turnaround Time:
Routine: 72 hours

Specimen Requirements:
Patient Preparation: None

Collect: Routine Venipuncture; 5-7 mL Heparinized Blood; Green-topped (heparin) vacutainer

Specimen Preparation: Must consult with Dr Schmalstieg prior to ordering test (409-772-9080); need a normal control drawn and sent at same time. Deliver immediately (cells die after 2-4 hours). Must specify antigen.

Storage/Transport Temperature: Deliver immediately, room temperature; Room C2.360, Research Building 006.

Stability: Deliver immediately (cells die after 2-4 hours)

Causes for Rejection: Unlabeled specimen, insufficient volume, clotted sample, sample too old

Clinical Information:
Must call ahead to schedule test, (409) 772-9081/9083.

Reference Range:
Physician analysis

Note: Must consult with Dr Schmalstieg prior to ordering test ((409) 772-9080);
Request Form: Clinical Immunology

CPT 4 Code:
86353

Review:
By Kimberly Palkowetz and 05/23/12

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

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