CSF - Lumbar Puncture
[ Common Collection Errors ] [ AutoCyte Prep ] [ Biopsy Tissue ] [ Blood Cultures ] [ Coagulation ] [ CSF ] [ Cytogenetics ] [ Cytopathology ] [ Fecal ] [ Pap Smears ] [ Throat Culture ] [ Urine Collection ] [ Viral ] [ Wound Sample ]
Clean the puncture site with
antiseptic solution and alcohol before needle insertion.
Insert a needle with stylet at
the L3-L4 or L4-L5 or L5-S1 interspaced. When the subarachnoid space is
reached, remove the stylet and spinal fluid will appear in the needle hub.
Slowly drain the CSF into the
sterile leak-proof tubes. Three separate tubes are generally required for
Microbiology, Hematology, and Chemistry testing. The second tube will
generally go to Microbiology and the third to Hematology. Specimens for
viral culture are sent on ice.