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ectocervix should be separately sampled.
sampled by rotating a wooden or plastic Ayre spatula with pressure.
with a pointed Ayre spatula rotating it 1-2 full turns (use of Cytobrush
enhances cell recovery).
Apply the material
rapidly to a glass slide labeled in pencil with the patient’s name and ID #;
Fix the slide
immediately by immersing it in 95% ethyl alcohol x 15 minutes, or spraying
with aerosol or pump fixative.
In older women, a
posterior vaginal pool or cervical canal aspiration should be taken to
detect endometrial lesions.
Collection of SurePath™ Thin Layer
Insert the Rover's
Cervex-Brush® into the endo-cervical canal.
pressure until the bristles form against the cervix.
pressure, hold the stem between the thumb and forefinger and rotate the
brush five times in a clockwise direction.
Remove the Rover's
Cervex-Brush® from the patient.
Placing your thumb
against the back of the brush, disconnect the entire brush from the stem,
dropping it into the CytoRich® preservative vial.
Place the cap on
the vial and tighten.
Label the vial
with patient's full name and ID number and/or physician name and date.
Cytopathology requisition form with the required information.
Put the vial and
the matching requisition into a specimen bag and send to the Cytopathology
Storage of Non-Gynecological
Body cavity fluids (pleural, abdominal, pericardial). Store
specimens in refrigerator (4°C - 8°C) - up to 48 hours. (During the regular
laboratory hours, specimens should be sent directly to Cytology Laboratory
Sputum, bronchial washings, bronchioalveolar lavage (BAL).
Store specimens in refrigerator (4°C - 8°C) - up to 48 hours. (During the
regular laboratory hours, specimens should be sent directly to Cytology
Spinal fluids, ventricular fluids, urine. Fix specimens
(fluids) with equal amount of 50% Alcohol and store in refrigerator (4°C -
8°C). (During the regular laboratory hours, no fixative should be added to
specimens. All specimens should be sent directly to Cytology Laboratory
Specimens collected during the weekends, holidays, and after
the regular hours can be delivered and stored in the refrigerator outside
the specimen receiving area of Cytopathology.
Reviewed 6/22/12 by Robert Stewart