Laboratory Test Directory

Rh IMMUNE GLOBULIN, human (007-0129)  "Rho(D) IMMUNE GLOBULIN, human"

Synonyms: Anti-Rh Globulin; RhoGAM^TM

CPT 4 Code: J2790

Test Order Mnemonic: BILL RHIG

Order Includes: Confirmation of Rh type on mother, fetal maternal hemorrhage screen, RhIG, Rho(D) immunoglobulin (IgG) containing anti-Rho(D) used to prevent the formation of Rh antibodies in the Rh negative individual who has received Rh positive blood as a result of carrying an Rh positive infant or transfusion of Rh positive blood.

Lab: Blood Bank

Request Form: Blood Bank Primary Request Form

Patient Preparation: Rosette test is to be done on postpartum maternal blood post-delivery to estimate the volume of fetal-maternal hemorrhage and the number of vials to administer.  Fetal Hemoglobin stain will be performed to confirm and quantify any positive rosette tests.  FMH and FHS testing is not required for Rh negative patients transfused with Rh positive platelets or red cells.

Collection: Collected postpartum.  Blood required from both mother and newborn.  Label specimens with patient's full name, UH # and date of collection.

Causes for Rejection: Newborn Rh negative, mother Rh positive or significant levels of anti-D present in mother's serum in the absence of antenatal RHIG administration, hemolysis, SST/serum separator tube, inadequate specimen identification, requisition not properly filled out.

Availability: Samples are accepted 24 hours per day at the Blood Bank lab, McCullough 5.102

Turnaround Time: Antenatal RHIG - 10 minutes; postpartum RHIG - 2 hours.

Special Instructions: Type and screen required during current hospital visit.

Specimen: Blood from both infant and mother

Volume: 5 mL blood from mother,  cord sample or venous heelstick from the newborn infant

Minimum Volume: 2 mL blood from mother; 1 mL from infant

Container: Lavender-topped tube (EDTA)

Reviewed/Revised 6/27/2010 by T. Moore, MT(ASCP)SBB