Rh IMMUNE GLOBULIN

Order Includes: Confirmation of Rh type on mother, fetal maternal hemorrhage screen, RhIG, Rho(D) immunoglobulin (IgG) containing anti-Rho(D) used to prevent the formation of Rh antibodies in the Rh negative individual who has received Rh positive blood as a result of carrying an Rh positive infant or transfusion of Rh positive blood.

Lab: Blood Bank

Request Form: Blood Bank Primary Request Form

Patient Preparation: Rosette test is to be done on postpartum maternal blood post-delivery to estimate the volume of fetal-maternal hemorrhage and the number of vials to administer. Fetal Hemoglobin stain will be performed to confirm and quantify any positive rosette tests.

Collection: Collected postpartum. Blood required from both mother and newborn. Label specimens with patient's full name, UH # and date of collection.

Causes for Rejection: Newborn Rh negative, mother Rh positive or significant levels of anti-D present in mother's serum in the absence of antenatal RHIG administration, hemolysis, SST/serum separator tube, inadequate specimen identification, requisition not properly filled out.

Availability: Samples are accepted 24 hours per day; Blood Bank John Sealy Annex., Room 1.180

Turnaround Time: Antenatal RHIG - 10 minutes; postpartum RHIG - 2 hours.

Special Instructions: Type and screen required during current hospital visit.

Specimen: Blood from both infant and mother

Volume: 7 mL blood from mother, capillary tubes from newborn heelstick or cord sample

Minimum Volume: 1 mL blood from mother; capillary tube from infant

Container: Lavender-topped tube (EDTA) from mother; capillary tube from baby.

Reviewed/Revised 02/14/06 by D. Madrigal, MT(ASCP)SBB

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