ANTIBODY SCREEN, Indirect (007-0047) "ANTIBODY SCREEN, Indirect"
Synonyms: Alloantibodies; Antibody Screen; Antiglobulin Test, Indirect; IAT; Indirect Antiglobulin Test; Indirect Anti-human Globulin Test; Indirect Coombs; Irregular Antibodies; Unexpected/Atypical/Irregular Antibodies
CPT 4 Code: 86850
Test Order Mnemonic: IAT
Test Includes: Indirect antiglobulin testing with screening cells. Use of panel cells when antibody identification is indicated. Dependent on initial panel results, cold autoadsorption, warm autoadsorption, selective cell panels, enzyme panels, and other specialized antibody identification procedures may be utilized to identify antibody(ies).
Lab: Blood Bank
Request Form: Blood Bank Division Primary Request Form
Causes for Rejection: Gross hemolysis, SST/serum separator tube, old specimen
Availability: Samples are accepted 24 hours per day at the Blood Bank lab, McCullough 5.102
Turnaround Time: 2 hours if negative; 8 hours or longer if positive
Special Instructions: All requests must include requesting physician’s name and ID number, patient's name, UH #, patient location, clinical information/diagnosis, identity of phlebotomist and date and time collected. Specimen must be labeled with patient full name, UH # and Date of Collection. Specimen must be transported to the laboratory in a biohazard specimen bag with request form in the pocket of the bag. Request form must be filled out completely and all information must be legible. Additional specimen may be requested if antibody identification is indicated.
Volume: 5 mL blood
Minimum Volume: 3 mL blood
Container: Lavender-topped tube (EDTA)
Reference Ranges: Negative
Test Method: Gel card
Reviewed/Revised 6/27/10 by T.Moore, MT(ASCP)SBB