Laboratory Test Directory

ANTIBODY SCREEN, Indirect (007-0047)  "ANTIBODY SCREEN, Indirect"

Synonyms: Alloantibodies; Antibody Screen; Antiglobulin Test, Indirect; IAT; Indirect Antiglobulin Test; Indirect Anti-human Globulin Test; Indirect Coombs; Irregular Antibodies; Unexpected/Atypical/Irregular Antibodies

CPT 4 Code: 86850

Test Order Mnemonic: IAT

Test Includes: Indirect antiglobulin testing with screening cells.  Use of panel cells when antibody identification is indicated.  Dependent on initial panel results, cold autoadsorption, warm autoadsorption, selective cell panels, enzyme panels, and other specialized antibody identification procedures may be utilized to identify antibody(ies).

Lab: Blood Bank

Request Form: Blood Bank Division Primary Request Form

Causes for Rejection: Gross hemolysis, SST/serum separator tube, old specimen

Availability: Samples are accepted 24 hours per day at the Blood Bank lab, McCullough 5.102

Turnaround Time: 2 hours if negative; 8 hours or longer if positive

Special Instructions: All requests must include requesting physician’s name and ID number, patient's name, UH #, patient location, clinical information/diagnosis, identity of phlebotomist and date and time collected.  Specimen must be labeled with patient full name, UH # and Date of Collection. Specimen must be transported to the laboratory in a biohazard specimen bag with request form in the pocket of the bag.  Request form must be filled out completely and all information must be legible.  Additional specimen may be requested if antibody identification is indicated.

Specimen: Blood

Volume: 5 mL blood

Minimum Volume: 3 mL blood

Container: Lavender-topped tube (EDTA)

Reference Ranges: Negative

Test Method: Gel card 

Reviewed/Revised 6/27/10 by T.Moore, MT(ASCP)SBB