Laboratory Test Directory

FETAL HEMOGLOBIN STAIN (007-0139)  "FETAL HEMOGLOBIN STAIN"

Synonyms: Kleihauer-Betke Stain, KB stain, Acid Elution Stain

CPT 4 Code: 85460

Test Order Mnemonic: FHS

Test Includes: The percentage of fetal cells in a maternal blood film is used to calculate the approximate volume of fetal-maternal hemorrhage.

Lab: Blood Bank

Request Form: Blood Bank Division Primary Request Form

Causes for Rejection: Gross hemolysis, serum separator tube, old specimen

Availability: Samples are accepted 24 hours per day at the Blood Bank lab, McCullough 5.102

Turnaround Time: 2 hours if negative; 2-4 hours may be required for confirmation of a positive test

Special Instructions: All requests must include requesting physician’s name and ID number, patient's name, UH #, patient location, clinical information/diagnosis, identity of phlebotomist and date and time collected.  Specimen must be labeled with patient's full name, UH # and Date of Collection. Specimen must be transported to the laboratory in a biohazard specimen bag with request form in the pocket of the bag.  Request form must be filled out completely and all information must be legible. 

Specimen: Blood

Volume: 5 mL blood

Minimum Volume: 3 mL blood

Container: Lavender-topped tube (EDTA)

Reference Ranges: Negative

Reviewed/Revised 6/27/2010 by T. Moore, MT(ASCP)SBB