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Department of Pathology

FOLATE, rbc (red blood cell) (087-0380)

CPT 4 Code: 82747

Test Order Mnemonic: RBC-FOLATE

Test Includes: Serum Folate, RBC Folate

Lab: Clinical Chemistry

Request Form: Special Chemistry Form 2

Collection: Routine venipuncture; AM collection is preferred. Test requires both SST and EDTA tubes.

Storage Instructions: SST (Serum Separator Tube): Centrifuge and refrigerate until delivered to lab. Remove serum from red cells and freeze serum if delivery delayed more than 24 hours.; Red-topped Tube: Centrifuge, remove serum from cells, refrigerate serum until delivered to lab. Freeze serum if delivery delayed more than 24 hours. Lavender-topped (EDTA preservative) Tube: DO NOT centrifuge; refrigerate until delivered to lab. Do not freeze lavender-topped tube; keep refrigerated.

Causes for Rejection: Gross hemolysis, Improper collection, improperly labeled, incomplete information, improper storage of sample. See special instructions.

Availability: Samples are received 24 hours per day at Sample Management, Room 5.136 McCullough.

Turnaround Time: 48 - 72 hours; test performed Monday - Friday.

Special Instructions: The test can be ordered only once per inpatient admission or once per month for outpatients. Additional requests must have the approval of the laboratory director.

Specimen: Blood

Volume: 7 mL blood preferred, 5 mL blood acceptable

Minimum Volume: 0.5 mL serum and entire volume of lavender-topped tube

Container Required: 7 mL SST and 5 mL lavender-topped (EDTA) tubes (see storage Instructions).

Reference Ranges: Serum: 1.6-12 ng/mL;    RBC Folate: 280-791 ng/mL

Test Method: Chemiluminescence

Reviewed/revised April 2007 by P. Harris

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