CPT 4 Code: 80170

Test Order Mnemonic: GENT T, GENT P or GENT R

Lab: Clinical Chemistry

Request Form: Special Chemistry Form 2

Collection: Routine venipuncture. Peak Levels: Collect blood 0.5 to 1.0 hour after the end of a 30-minute infusion or one hour after IM dose; Trough Levels: Draw immediately before dosing.

Storage Instructions: Refrigerated (2-8C) as soon as possible. Centrifuge the specimen, remove the serum from the red cells and refrigerate.

Causes for Rejection: Hemolysis, improperly labeled,   blood collected in a SST/gel barrier tube or any anticoagulated tube. See special instructions.

Availability: Samples are received 24 hours per day at Sample Management, Room 5.136 McCullough.

Turnaround Time: Routine: 24 hours; STAT: 1 hour

Special Instructions: If the sample is obtained through the infusion line, flush the line thoroughly with saline before taking the blood sample.

Specimen: Blood

Volume: 3 mL blood

Minimum Volume: 0.5 mL serum

Container: Red-topped tube. Do not use SST/gel separator tube.

Reference Ranges: Therapeutic: Peak range, 2-8mcg/mL; Trough range, 0.5-2.0 mcg/mL.

Critical Values: Potential Toxicity: Peak >10 mcg/mL; Trough >2 mcg/mL

Test Method: Chemiluminescence

Reviewed/revised April 2007 by P. Harris