GLUCOSE TOLERANCE, serum or plasma

Storage Instructions: Centrifuge the specimen to separate serum from cells within 2 hours of collection; if using grey-topped tubes, plasma need not be separated if specimens are delivered to the laboratory within 8 hours of collection. Refrigerate samples at 2-8°C

Causes for Rejection: Gross hemolysis, improperly labeled specimens, collection time not written on tubes or urine containers. See special instructions.

Availability: Samples are received 24 hours per day at Sample Management, Room 5.136 McCullough.

Turnaround Time: Routine: 4 hours; STAT: 1 hour

Special Instructions: All specimens must have collection time on tube and urine container (e.g., 1/2 hr, 1 hr, 2 hr, 3 hr, etc.). Write times of collection for each sample on the request form.

Specimen: Blood and urine

Volume: 7 mL blood + 10 mL urine

Minimum Volume: 0.5 mL serum or plasma + 5 mL urine

Container: SST (gel barrier) or gray-topped ( sodium fluoride) tube for blood sample and plastic container for urine aliquot

Reference Ranges:

Analyte

Time Intervals

Reference Range

Glucose Levels

30 minutes

1 hour

90 minutes

110-170 mg/dL

120-170 mg/dL

100-170 mg/dL

Glucose Tolerance Test

2 hours

3 hours

4 hours

5 hours

6 hours

7 hours

8 hours

70-120 mg/dL

70-110 mg/dL

Test Method: End Point Colorimetric, oxidase

Reviewed/revised April 2007 by P. Harris

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