IMMUNOELECTROPHORESIS, Serum and Urine

Test Includes: Serum: Total Protein, Albumin, IgG, IgA, IgM, High Resolution electrophoresis, Interpretation and immunofixation as needed. Urine: Qualitative Protein, High Resolution Electrophoresis, interpretation and Immunofixation as needed.

Lab: Immunochemistry

Request Form: Clinical Immunology

Collection: Routine venipuncture; Urine: 24 hour collection or random collection

Storage Instructions: Prefer prompt delivery. Blood - seperate serum from cells and refrigerate. Urine - refrigerate.

Causes for Rejection: Gross hemolysis, urine sample collected with any preservative

Availability: Samples are received 24 hours per day at Sample Management, Room 5.138, McCullough.

Turnaround Time: Routine: 48 hours; test is performed Monday - Friday

Special Instructions: Blood sample is always require in conjunctin with Urine requrest and must be collected within 24 hours of each other. Record 24-hour urine volume on request formif aliquot is sent to laboratory.

Specimen: Blood and urine

Volume: 7 mL blood ; Urine: Prefer entire amount of urine collected; 20 mL aliquot of well-mixed 24 hour urine or 10 mL or random urine acceptable

Minimum Volume: 3 mL blood or 5 mL urine

Container: SST (gel barrier) preferred; red-topped tube acceptable; Urine: 3 liter brown plastic jug with no preservative for 24-hour sample or sterile 4 oz plastic container with screw cap for random sample.

Reference Ranges: Serum: Total Protein 6.0-8.0 g/dL; Albumin: 3.2-5.2 g/dL; IgG, IgA, IgM; See individual immunoglobins
Urine: Protein, qualitative, negative

Test Method: Immunoelectrophoresis

Reviewed/revised April 2007 by P. Harris

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