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IMMUNOELECTOPHORESIS, SERUM AND URINE (087-0242) Synonyms: IEP CPT 4 Code: 86320 Test Order Mnemonic: IEP SER+UR Test Includes: Serum: Total Protein, Albumin, IgG, IgA, IgM, High Resolution electrophoresis, Interpretation and immunofixation as needed. Urine: Qualitative Protein, High Resolution Electrophoresis, interpretation and Immunofixation as needed. Lab: Immunochemistry Request Form: Clinical Immunology Collection: Routine venipuncture; Urine: 24 hour collection or random collection Storage Instructions: Prefer prompt delivery. Blood - seperate serum from cells and refrigerate. Urine - refrigerate. Causes for Rejection: Gross hemolysis, urine sample collected with any preservative Availability: Samples are received 24 hours per day at Sample Management, Room 5.138, McCullough. Turnaround Time: Routine: 48 hours; test is performed Monday - Friday Special Instructions: Blood sample is always require in conjunctin with Urine requrest and must be collected within 24 hours of each other. Record 24-hour urine volume on request formif aliquot is sent to laboratory. Specimen: Blood and urine Volume: 7 mL blood ; Urine: Prefer entire amount of urine collected; 20 mL aliquot of well-mixed 24 hour urine or 10 mL or random urine acceptable Minimum Volume: 3 mL blood or 5 mL urine Container: SST (gel barrier) preferred; red-topped tube acceptable; Urine: 3 liter brown plastic jug with no preservative for 24-hour sample or sterile 4 oz plastic container with screw cap for random sample. Reference Ranges: Serum: Total Protein 6.0-8.0
g/dL; Albumin: 3.2-5.2 g/dL; IgG, IgA, IgM; See individual immunoglobins Test Method: Immunoelectrophoresis Reviewed/revised April 2007 by P. Harris
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