IMMUNOELECTOPHORESIS, SERUM AND CSF (087-0242)

Synonyms: IEP

CPT 4 Code: 86320

Test Order Mnemonic: IEP S+CSF

Test Includes: Serum: Total Protein, Albumin, IgG, IgA, IgM, High Resolution electrophoresis, Interpretation and immunofixation as needed.  CSF:  Albumin, IgG, IgA, IgM, IgG Index, High Resolution Electrophoresis and interpretation

Lab: Immunochemistry

Request Form: Clinical Immunology

Collection: Routine venipuncture; CSF - routine lumbar puncture

Storage Instructions: Prefer prompt delivery.  Blood - seperate serum from cells and refrigerate.  CSF - refrigerate.

Causes for Rejection: Gross hemolysis, lipemic serum

Availability: Samples are received 24 hours per day at Sample Management, Room 5.138, McCullough.

Turnaround Time: Routine: 48 hours; test is performed Monday - Friday

Special Instructions:  Blood sample is always require in conjunctin with CSF requrest and must be collected within 24 hours of each other.

Specimen: Blood or CSF

Volume: 7 mL blood or 3 mL CSF

Minimum Volume: 3 mL blood or 1 mL CSF

Container: SST (gel barrier) preferred;  red-topped tube acceptable; CSF: 7 mL sterile plastic tube with screw cap

Reference Ranges: Serum:   Total Protein 6.0-8.0 g/dL; Albumin: 3.2-5.2 g/dL; IgG, IgA, IgM; See individual immunoglobins
CSF:  Albumin, 10-30 mg/dL; IgG, <6.1 mg/dL, IgA, <1.1 mg/dL; IgM, <1.1 mg/dL; IgG index, <0.58

Test Method: Immunoelectrophoresis

Reviewed/revised April 2007 by P. Harris