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Department of Pathology

LACTATE DEHYDROGENASE ISOENZYMES (087-0233 and 087-0264)

Synonyms: LDH isoenzymes

CPT 4 Code: 83615 and 83625

Test Includes: LD1, LD2, LD3, LD4, LD5, LD1/LD2 Ratio, and Interpretation

Lab: Clinical Chemistry

Request Form: Special Chemistry Form 2

Collection: Routine venipuncture, random urine, or body fluid aspiration

Storage Instructions: Blood: Centrifuge the specimen, remove serum from the red cells and store serum at 2-8°C if not delivered to the lab within 4 hours after collection. Refrigerate urine and body fluids.

Causes for Rejection: Hemolysis. See special instructions.

Availability: Samples are received 24 hours per day at Sample Management, Room 5.136 McCullough.

Turnaround Time: 24 -48 hours

Special Instructions: Test is available through a consultation with laboratory director only. For assessment of myocardial infarction, see CK-MB and Troponin.

Specimen: Blood, urine, or body fluid

Volume: 7 mL blood, 7 mL urine, or 2 mL body fluid

Minimum Volume: 0.5 mL serum, 0.5 mL urine, or 0.5 mL body fluid

Container: Blood: SST (gel barrier tube) or red-topped tube; Urine: plastic urine cup; Body Fluid: plastic, conical, capped body fluid tube

Reference Ranges: Serum: LD1 = 19-30%, LD2 = 32-48%, LD3 = 12-22%, LD4 = 5-11%, LD5 = 5-13%, LD1/LD2 = < 0.83; fluids and urine have no reference ranges

Reviewed/revised April 2007 by P. Harris

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