CPT 4 Code: 80202

Test Order Mnemonic: VANCO T (trough), VANCO P (peak), VANCO R (random)

Lab: Clinical Chemistry

Request Form: Special Chemistry Form 2

Collection: Routine venipuncture. Draw trough levels immediately before dose; peak levels 0.5 to 1.0 hour after the end of a 30- to 60-minute infusion or one hour after IM dose.

Storage Instructions: Serum and blood samples should be refrigerated as soon as possible. If analysis is delayed more than 8 hours, centrifuge the specimen, remove the serum and freeze the serum (-20C).

Availability: Samples are received 24 hours per day at Sample Management, Room 5.136 McCullough.

Turnaround Time: Routine: 24 hours; STAT 1 hour

Special Instructions: If sample is obtained through the infusion set, flush the line thoroughly with saline before taking the blood sample. Request form must contain date and time of collection and time of last dose.

Causes for Rejection: Blood drawn in SST/gel separator tubes or any anticoagulated tube.

Specimen: Blood

Volume: 3 mL blood

Minimum Volume: 0.5 mL serum

Container: Red-topped tube

Reference Ranges: Therapeutic Range: Peak range, 30-40 mcg/mL; Trough range, 5-10 mcg/mL

Critical Values: Potential Toxicity: Peak, >50 mcg/mL; Trough, >15 mcg/mL

Test Method: Chemiluminescence

Reviewed/revised April 2007 by P. Harris