Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by
collecting a discard tube prior to collecting the
blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube
should also be collected prior to collection of the blue
top to ensure sufficient sample volume. Drawing a
discard tube will displace the air from the blood
collection set tubing to ensure proper blood draw
For patients on heparin therapy, the whole blood sample
must be centrifuged within 1 hour of collection.
up to 4 hours
after draw, at ROOM TEMPERATURE (20 +/- 5░C).
If delivery time is to be greater than 4 hours from time
centrifuge the specimen for 15 minutes at 2000-2500g.
Separate the plasma from the cells and freeze at -200C.
4 hrs at 20 +/- 5░C.
Patients on heparin therapy -plasma is stable for 2 hrs
at 20 +/- 5░C when
collected in 3.2% sodium citrate (blue top). Frozen
plasma 2 Weeks at -20░C. Whole blood is stable for 4
hours at room temperature.
QNS, clotted, severely hemolyzed, greater than 4 hours
wrong tube (3.8% sodium citrate), high hematocrit (>
sample identification error, processed and/or stored