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BUFFY COAT SMEARS: (089-3172)

Clinical Indication:

A buffy coat preparation facilitates the search for immature and abnormal white blood cells, particularly when the peripheral blood count is low, and in identification of bacteria or yeast in septic and immunocompromised or immunosuppressed patients.

Test Mnemonic:

 BUFFYCOAT

Methodology:

 Micro-hematocrit centrifugation of specimen.  Concentration of WBC's; Wright-Giemsa Stain.

Performed:

 Samples are accepted 24 hours per day at Sample Management, Room 5.136 McCullough

Turnaround Time:

 48 hours

Specimen Requirements:

Collect: Routine venipuncture, EDTA (Lavender-top tube)

Volume: 5 ml

 

Minimum Volume: 3 ml

Storage/Transport: Stable at Room Temperature for 24 hours. 

Stability: Stable Refrigerated for 36 hours.

Causes for Rejection: Clotted, QNS, old specimen, identification errors, labeling errors, inappropriate anticoagulants

Clinical Information:

Test performed as consult with Hematology Staff.

Reference Range:

Interpretive Test (call Lab)

CPT 4 Code:

 85009

Reviewed: 12-27-2011

 A. Huerta, BS, MT(ASCP), MBA

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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