CBC - without DIFFERENTIAL

Test Includes: White blood cell count (WBC), red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), platelet count (PLT), mean Platelet volume (MPV)

Applies To: Monitoring hematological parameters when WBC differential and white/red blood cell morphology is not required.

Request Form: Hematology A

Collection: Venipuncture preferred; Micro-collection acceptable.

Storage Instructions: Stable at room temperature for 24 hours. Stable refrigerated for 36 hours..

Cause For Rejection: Old specimen, clotted, QNS, hemolysis, identification errors

Availability: Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough

Turnaround Time: STAT: 1 hour; Routine: 4 hours

Special Instructions: ECMO specimens should be delivered directly to the Hematopathology lab (5.142 McCullough)

Specimen: Blood

Volume: 2.0 ml

Minimum Volume: 1 mL (0.5 mL in extreme emergency)

Container: EDTA (Lavender-top tube)

Reference Ranges: Age/Sex Related (See patient chart)

Critical Values: WBC £ 2.0 x 10³/mL; Hemoglobin £ 7.0 g/dL (infants), £5.0 g/dL (children/adult); Hematocrit : <21% (Infants), £15.0% (children/adult), Platelets £ 50 or > 1000 x 10³/mL

Test Method: Automated multi-parameter counting of red blood cells (RBC) and platelets using electronic resistance detection enhanced by hydrodynamic focusing. The hematocrit is measured utilizing cumulative pulse height detection. Hemoglobin is measured using a sodium lauryl sulfate method. WBC count, and nucleated red blood cells are evaluated using flow cytometry.

Interfering Substances: Clots or fibrin strands; inappropriate anticoagulant; extremely elevated WBC counts; nucleated red blood cells; sickle cells; clumped platelets; giant platelets, circulating micro-megakaryocytes; hemolysis; electrolyte imbalances; warm or cold agglutinins; elevated plasma lipids; elevated chylomicrons; elevated bilirubin; elevated serum urea nitrogen,

Revised/Reviewed 10/10/07 by L. Emanuel, MT(ASCP)

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