Laboratory Test Directory

COMPLETE BLOOD COUNT  WITHOUT DIFFERENTIAL (089-0256)

Clinical Indication:

Monitoring hematological parameters when WBC differential and white/red blood cell morphology is not required.

Test Mnemonic:

 HH

Methodology:

Automated, multi-parameter counting of red blood cells (RBC) and platelets using electronic resistance detection enhanced by hydrodynamic focusing. The hematocrit is measured utilizing cumulative pulse height detection. Hemoglobin is measured using a sodium lauryl sulfate method. WBC count, and nucleated red blood cells are evaluated using flow cytometry.

Performed:

 Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough

Turnaround Time:

 STAT: 1 hour; Routine: 4 hours

Specimen Requirements:

Special Instructions: ECMO specimens should be delivered directly to the Hematopathology lab (5.142 McCullough)

Collect: Routine venipuncture; Collect in EDTA (lavender tube). Micro-collection is acceptable.  The specimen should be collected to the appropriate fill volume stated on the collection tube.  The minimum volume required is 90% of the stated tube volume.

Storage/Transport:  Room Temperature 4 hours.  If testing cannot be completed within 4 hours sample should be refrigerated at 2-8° C.

Stability: Stable Refrigerated for 24 hours.

Causes for Rejection:  Clotted, QNS, identification error, hemolysis.

Clinical Information:

Critical Values:

WBC< 2.0 x 10³/mL

Hemoglobin <7.0 g/dL (infants),  <5.0 g/dL children/adult)

Hematocrit : <21% (Infants),  <15.0% (children/adult)

Platelets  <50 or > 1000 x 10³/mL

Interfering Substances: Clots or fibrin strands; inappropriate anticoagulant; extremely elevated WBC counts; nucleated red blood cells; sickle cells; clumped platelets; giant platelets, circulating micro-megakaryocytes; hemolysis; electrolyte imbalances; warm or cold agglutinins; elevated plasma lipids; elevated chylomicrons; elevated bilirubin; elevated serum urea nitrogen.

Reference Range:

By report (reports may vary based on instrumentation, patient age and sex)

CPT 4 Code:

 85027 Limited Coverage Test (NCD)

Reviewed: 8/15/12

 A. Huerta, BS, MT(ASCP), MBA

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.