CD4/CD8 (089-3195)

Synonyms:   CD4; CD8

CPT Code(s):   86360 (CD4/CD8); 86361 (if ordered as only CD4)

Test Order Mnemonic:   CD4CD8; CD4

Lab:   Flow Cytometry

Test Includes:   CD4%, CD8% (when ordered as CD4CD8), CD4/CD8 ratio (when ordered as CD4CD8), Absolute CD4#, Absolute CD8# (when ordered as CD4CD8)

Applies To:   Aids in monitoring the lymphocyte subsets of patients with immune deficiency diseases, autoimmune disease or immune reactions.

Request Form:   Hematology B

Collection:   Routine venipuncture

Storage Instructions:   Store at room temperature (18-22°C). DO NOT REFRIGERATE.

Cause For Rejection:   Clotted, QNS, hemolysis, identification error, specimen refrigerated, specimens received on weekend and holidays, specimen received after noon cut off on Fridays.

Availability:   Samples are accepted in Sample Management, 5.136 McCullough,
Monday-Thursday (8AM-5PM). Friday cut-off time is noon.

Turnaround Time:   48 hours

Specimen:   Blood

Volume:   4 ml

Minimum Volume:   1 ml

Container:   EDTA (Lavender-top tube); pediatric tube acceptable

Reference Ranges:

Test Methodology:   Flow cytometry

Interfering Substances:   Cephalosporins; elevated lipids, Zidovudine (AZT), Nicotine, Corticosteroids, diurnal variation

Reviewed 4/14/08, by J Patel, MT (ASCP)