Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by
collecting a discard tube prior to collecting the
blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube
should also be collected prior to collection of the blue
top to ensure sufficient sample volume. Drawing a
discard tube will displace the air from the blood
collection set tubing to ensure proper blood draw
mL or 1.8 mL blood, ( blue top tube must be full)
Whole blood is viable for 4 hours at room
temperature. If time from draw to receipt in the
laboratory is to be greater than 4 hours,
centrifuge the specimen for 15 minutes at 1500g and
separate the plasma from the cells.
plasma to 12x75 plastic tube
Cap tube and re-spin
for 15 minutes at 1500g
remove from centrifuge without disturbing any
platelets and/or cell pellets that might be on the
bottom or sides of the tube.
Carefully transfer plasma into freezer tubes
(plastic screw-top cryo-tubes) for testing or
Whole blood 4 hours at room temperature, Plasma 4 hrs at
2-4°C. Frozen plasma 2 weeks stored at -20°C.
Causes for Rejection:
QNS, clotted, severely hemolyzed, specimen greater than
4 hours old, wrong tube (3.8% sodium citrate), sample
identification error, sample processed/transported
and/or stored improperly.