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ESTERASE STAIN (089-3166) Synonyms: Specific Esterase, Non-Specific Esterase, Dual Esterase, Combined Esterase CPT 4 Code: 88319 Test Order Mnemonic: ESTER Lab: Hematopathology Test Includes: Non-specific esterase and specific esterase stains Collection: EDTA (Lavender-top tube). Invert promptly to prevent clotting. Storage Instructions: Deliver specimen to Hematopathology Lab (5.142 McCullough) within 30 minutes. Cause For Rejection: QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangement made. Availability: Samples are accepted 8 AM - 2 PM, Monday-Friday, Room 5.142, McCullough. Turnaround Time: 48 hours Special Instructions: Test must be approved by Hematopathologist or Hematology/Oncology Faculty Applies To: Identification of specific leukemias, particularly monocytic vs. myelocytic. Request Form: Hematology A Specimen: Blood, Bone Marrow aspirate Volume: 2.0 ml Minimum Volume: 1.0 ml Container: EDTA (Lavender-top tube) Reference Range: Interpretive Test (call Lab) Test Method: Cytochemical hydrolysis of ester linkages using naphthol AS-D chloroacetate esterase and a -naphthyl acetate esterase; Miscroscopy. Interfering Substances: Inappropriate fixatives or anticoagulants, clotted specimen, inadequate spicules. Revised 10/10/07 by L. Dornak,MT(ASCP) |
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