ESTERASE STAIN (089-3166)

Synonyms: Specific Esterase, Non-Specific Esterase, Dual Esterase, Combined Esterase

CPT 4 Code:  88319

Test Order Mnemonic: ESTER

Lab: Hematopathology

Test Includes: Non-specific esterase and specific esterase stains

Collection: EDTA (Lavender-top tube). Invert promptly to prevent clotting.

Storage Instructions: Deliver specimen to Hematopathology Lab (5.142 McCullough) within 30 minutes.

Cause For Rejection: QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangement made.

Availability: Samples are accepted 8 AM - 2 PM, Monday-Friday, Room 5.142, McCullough.

Turnaround Time: 48 hours

Special Instructions: Test must be approved by Hematopathologist or Hematology/Oncology Faculty

Applies To: Identification of specific leukemias, particularly monocytic vs. myelocytic.

Request Form: Hematology A

Specimen: Blood, Bone Marrow aspirate

Volume: 2.0 ml

Minimum Volume: 1.0 ml

Container: EDTA (Lavender-top tube)

Reference Range: Interpretive Test (call Lab)

Test Method: Cytochemical hydrolysis of ester linkages using naphthol AS-D chloroacetate esterase and a -naphthyl acetate esterase; Miscroscopy.

Interfering Substances: Inappropriate fixatives or anticoagulants, clotted specimen, inadequate spicules.

Revised 10/10/07 by L. Dornak,MT(ASCP)