Patient must not be on any anticoagulation therapy for 48 hours
before blood collection.
Routine venipuncture; discard 1st mL of blood or collect other
tubes (EDTA, red-topped) prior to collecting sample in (2)
blue-topped (3.2% sodium-citrate) tubes.
If drawing from a blood collection set, draw a discard tube.
This will displace the air from the blood collection set tubing
to ensure proper blood draw volume. Pulling in this air will
result in under filling the tube, thus causing an incorrect
after draw, at ROOM TEMPERATURE.
If delivery time is to be greater than 4 hours from time of
centrifuge the sample at 2000 - 2500g for 10 minutes.
Transfer plasma to 12x75 plastic tube
Cap tube and re-spin
at 2000 - 2500g for 10 minutes
remove from centrifuge without disturbing any platelets and/or
cell pellets that might be on the bottom or sides of the tube.
Carefully transfer plasma into freezer tubes (plastic screw-top
cryo-tubes) for testing or freezing .
not keep plasma at 2-80C because the factor VII can
be activated by kallikrein system in this temperature range.
Whole blood 4 hours at room temperature. Plasma is stable for
4 hours at 20+
50C. Frozen plasma 1 month at -20C.
Causes for Rejection:
clotted, severely hemolyzed specimen, specimen greater than 4
hours old, wrong tube (3.8% sodium citrate), high hematocrit (>
55%), sample identification error, sample processed/transported,
and/or stored improperly.