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Factor VIII Assay: (089-0010)
Clinical Indication:

 Measures levels of factor/s that can be useful in diagnosing bleeding disorders and liver disease.
Test Mnemonic:

 FACTORVIII
Methodology:

  Electromagnetic Viscosity Detection
Performed:

 Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough Bldg.

Turnaround Time:

 STAT: 1 1/2 hours; ROUTINE: 4 hours
Specimen Requirements:

Patient Preparation: Patient must not be on any anticoagulation therapy for 48 hours before blood collection.

Collect: Routine venipuncture; discard 1st mL of blood or collect other tubes (EDTA, red-topped) prior to collecting sample in  (2) blue-topped (3.2% sodium-citrate) tubes.

Specimen Preparation: Samples should be received in laboratory within ˝ hour of draw for best results.  Acceptable up to4 hours after draw, at ROOM TEMPERATURE.

Storage/Transport: NOTE: If delivery time is to be greater than 4 hours from time of draw, centrifuge the specimen for 15 minutes at 2000-2500g.  Separate the plasma from the cells and snap freeze (-200C) the plasma.

Stability:Plasma 4 hours at 20+/- 5°C. Frozen 15 days at -20°C. Whole blood 4 hours at room temperature.

Causes for Rejection: QNS, clotted, severely hemolyzed specimen, specimen greater than 4 hours old, wrong tube (3.8% sodium citrate), high hematocrit (> 55%), identification error, sample processed/transported, and/or stored improperly
Clinical Information:

Interfering substances:  oral contraceptives, anticoagulant therapy
Reference Range:

0.47 - 1.70 Units
CPT 4 Code:

 85240
3/27/2012 Reviewed:  M. Abad, MLS (ASCP)CM, SH

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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