Patient must not be on any anticoagulation therapy for
48 hours before blood collection.
Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by
collecting a discard tube prior to collecting the
blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube
should also be collected prior to collection of the blue
top to ensure sufficient sample volume. Drawing a
discard tube will displace the air from the blood
collection set tubing to ensure proper blood draw
Samples should be received in laboratory within ˝ hour
of draw for best results.
Acceptable up to
after draw, at ROOM TEMPERATURE.
If delivery time is to be greater than 4 hours from time
centrifuge the sample for 10 minutes at 2500g to ensure
platelet poor plasma.
Transfer plasma to 12x75 plastic tube
Cap tube and re-spin
for 10 minutes at 2500g
remove from centrifuge without disturbing any platelets
and/or cell pellets that might be on the bottom or sides
of the tube.
Carefully transfer plasma into freezer tubes (plastic
screw-top cryo-tubes) for testing or freezing .
Whole blood 4 hours at room temperature. Plasma stable
for 4 hours at 20+/- 50C. 6 hours at 2-80C.
Do not store at 2-8 0C if factor VII will
also be tested. Factor VII may be activated by this
temperature range. Frozen plasma stable for 1 week at
Causes for Rejection:
QNS, clotted, severely hemolyzed specimen, specimen
greater than 4 hours old, wrong tube (3.8% sodium
citrate), high hematocrit (> 55%), sample
identification error, sample processed/transported,
and/or stored improperly.