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FACTOR II ASSAY: (089-0005)
Clinical Indication:

 Measures levels of factor/s that can be useful in diagnosing bleeding disorders and liver disease
Test Mnemonic:

FACTORII
Methodology:

 Electromagnetic Viscosity Detection
Performed:

 Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough Bldg.

Turnaround Time:

 STAT: 1 1/2 hours; ROUTINE: 4 hours
Specimen Requirements: Patient Preparation: Patient must not be on any anticoagulation therapy for 48 hours before blood collection.

Collect: Routine venipuncture; discard 1st mL of blood or collect other tubes (EDTA, red-topped) prior to collecting sample in  (2) blue-topped (3.2% sodium-citrate) tubes.

Specimen Preparation: Samples should be received in laboratory within ˝ hour of draw for best results.  Acceptable up to 4 hours after draw, at ROOM TEMPERATURE.

Specimen: Blood

Volume: 2.7ml (need 2 tubes)

Minimum Volume: Call Lab

Storage/Transport:  NOTE: If delivery time is to be greater than 4 hours from time of draw, centrifuge the sample for 10 minutes at 2500g. Separate the plasma from the cells and snap freeze (-200C) the plasma.

Stability:  Plasma 4 hours at 20+/-  50C.  8 hours at 2-8 0C. If the plasma is to be used for testing factor VII, do not store at 2-8 0C because the factor VII may be activated in that temperature range.  Whole blood for 4 hours at room temperature.

Causes for Rejection: QNS, clotted, severely hemolyzed specimen, specimen greater than 4 hours old, wrong tube (3.8% sodium citrate), high hematocrit (> 55%), identification error, sample processed/transported, and/or stored improperly.
Clinical Information:

Interfering substances:  oral contraceptives, anticoagulant therapy
Reference Range:

0.80 - 1.27 Units
Note:

 
CPT 4 Code:

 85210
12-5-2011 Reviewed:  M. Abad, MLS (ASCP)CM, SH

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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