FLOW CYTOMETRY MARKERS: (089-0533)

 Determination of Leukocyte Markers

 FLOWPHENO

 Flow Cytometry

 Samples are accepted in Hematopathology Monday-Friday (call lab extension 25111 for Friday deadline), Room 5.142 or 5.158 McCullough.

 48-72 hours

Collect:  Blood and Bone Marrow specimens should be in EDTA (Lavender-top tube).  Blood may be collected in heparin (Green-top tube).  Tissue Samples should be placed in RPMI culture media.  FNA fluid may be collected in RPMI culture media. CSF and other body fluids may be collected into standard CSF collection tubes.

Volume:  4 ml for blood

Minimum Volume: 10 ml for body fluid. Minimal volume also depends on cellularity (minimally, 10,000 cells are required for flow). Call Hematology lab.

Specimen Preparation:  Consult information should include name/phone or beeper number of ordering physician, and provisional diagnosis.

Storage/Transport:  Blood and bone marrow specimens refrigerated at -4 °C or at room temperature (18-22°C); Blood and bone marrow must be received within 24 hours of collection.  FNA and body fluids should be received as soon as possible within 6 hours of collection and must be refrigerated.

Stability: For blood and bone marrow: refrigerated or at room temperature: 48 hours, frozen: unacceptable; for FNA and body fluids: refrigerated: 48 hours, ambient and frozen: unacceptable.

Causes for Rejection: Clotted, QNS, hemolyzed, received >24 hours after collection (>6 hours for body fluids other than blood and bone marrow), identification errors, frozen specimens, specimens received on Saturday or Sunday (emergent cases will be sent out to ARUP on weekends with approval from the Hematopathology Director).

By report (reports may vary based on instrumentation, patient age and sex)

 88184 (first marker);  88185 (each additional marker)

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

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