Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by collecting a
discard tube prior to collecting the blue-topped (3.2%
sodium-citrate) tube. For collections with butterfly blood
collection sets, a discard tube should also be collected prior
to collection of the blue top to ensure sufficient sample
volume. Drawing a discard tube will displace the air from the
blood collection set tubing to ensure proper blood draw volume.
Samples should be collected and centrifuged as soon as possible
after collection and within 60 minutes.
If delivery time is to be greater than 1 hour from time of
collection, double spin the specimen Centrifuge for 10 minutes
at 1,500g. Transfer the plasma to a plastic centrifuge tube and
centrifuge again for 10 minutes at 1,500g. After the second
centrifugation, transfer the plasma into another tube for
testing leaving approximately 1 mL of plasma in tube to ensure
all platelets have been removed.
Whole blood 1 hour. Plasma
4 hrs at 20 +/- 5°C.
Causes for Rejection:
clotted, severely hemolyzed specimen, specimen greater than 4
hours old, wrong tube (3.8% sodium citrate), high hematocrit (>
55%), sample identification error, sample processed/transported,
and/or stored improperly.