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HEMOGLOBIN ELECTROPHORESIS (089-3106) Synonyms: Hgb-Elect, Hb-Elect CPT 4 Code: 83020 Test Order Mnemonic: HGBELECT Test Includes: Sickling test, hemoglobin identifications, hemoglobin quantitations, hemoglobin F%, hemoglobin A2%, interpretation of hemoglobinopathy by Pathologist. Applies To: Aids in diagnosis of patients presenting with certain hematological abnormalities (i.e., thalassemia, anemias, abnormal RBC morphology) Lab: Hematopathology Request Form: Hematology B Patient Preparation: If patient is to receive RBC transfusion, collect before transfusion. Collection: Routine venipuncture Storage Instructions: Specimens not delivered to the laboratory within 2 hours should be refrigerated (stored at 2 - 8°C). Cause For Rejection: Collected in wrong tube, clotted, identification error Availability: Samples are accepted 24 hours per day Sample Management, 5.136 McCullough. Turnaround Time: Routine testing is batched on Tuesday/Thursday. Maximum 7 day turnaround time. STAT testing is available ONLY if needed prior to RBC transfusion. STAT turnaround time is 4 hours, Monday-Friday if received BEFORE 2 pm. STAT testing is not available on weekends or holidays. Specimen: Blood Volume: Pediatric: 1-3 ml; Adults: 5-10 ml Container: preferred EDTA (Lavender-top tube) ; accepted Heparin (Green-top tube) Reference Ranges: Hemoglobin A2 = 1.3-3.3%; Hemoglobin F% £ 1.5 Test Methods: Test Battery Includes tube solubility (Sickle Screen) and High Performance Liquid Chromatography (HPLC); with Isoelectric Focusing (IEF): Microchromatography; Alkali Denaturation and Citrate or Cellulose Electrophoresis when indicated. Interfering Substances: Transfusions Reviewed 10/17/2006 by L. Hinson, MT(ASCP) |
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