Laboratory Test Directory

LUPUS PANEL: (089-0106),(089-0220)

(089-0526),(089-0526)

Clinical Indication:

Detects presence of Lupus Anticoagulant

Test Mnemonic:

LUPUSPANEL

Methodology:

 Electromechanical viscosity detection.

Performed:

 Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough Bldg.

Turnaround Time:

 Test performed once per week (batch); maximum 7 day turn around.  Call Laboratory for schedule.

Specimen Requirements:

Collect:  Blue-topped (3.2% sodium-citrate) tube. Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume.

Specimen Preparation: Acceptable up to 4 hours after draw, at ROOM TEMPERATURE.

Storage/Transport: Whole blood is viable for 4 hours at room temperature.   If time from draw to receipt in the laboratory is to be greater than 4 hours, centrifuge the specimen for 15 minutes at 1500g and separate the plasma from the cells.

·         Transfer plasma to 12x75 plastic tube

·         Cap tube and re-spin  for 15 minutes at 1500g

·         CAREFULLY remove from centrifuge without disturbing any platelets and/or cell pellets that might be on the bottom or sides of the tube.

·         Carefully transfer plasma into freezer tubes (plastic screw-top cryo-tubes) for testing or freezing .

Stability Whole blood 4 hours at room temperature, Plasma 4 hrs at 2-4°C. Frozen plasma 2 weeks stored at -20°C.

Causes for Rejection: 3.8% sodium citrate tube, QNS, clotted, severely hemolyzed specimen, specimen greater than 4 hours old, sample identification error, sample processed and/or stored improperly.

Clinical Information:

If applicable, state clinical information that is required to be provided with specimen.

Reference Range:

By report (reports may vary based on instrumentation, patient age and sex)

CPT 4 Code:

85610 (PT); 85730 (APTT); 85613 (DRVVT), 85335 (Inhibitor screen)

 Reviewed: 3/20/13

W.Russo, MT(ASCP)

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.