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Pathology Clinical Services

Department of Pathology

PERIODIC ACID-SCHIFF STAIN (089-3163)

Synonyms: PAS

CPT 4 Code:  88312

Test Order Mnemonic: PAS

Applies To: Identification of certain leukemias, particularly Erythroleukemia and Sezary Syndrome

Lab: Hematopathology

Request Form: Hematology A

Collection: Collect in EDTA. Invert promptly to prevent clotting.

Storage Instructions: Deliver specimen to Hematopathology within 30 minutes of collection.

Cause For Rejection: QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangement made

Availability: Samples are accepted from 8AM to 2PM, Monday-Friday in Room 5.142, McCullough Building.

Turnaround Time: 48 hours

Special Instructions: Test must be approved by Hematopathologist or Hematology/Oncology Faculty member.

Specimen: Blood, bone marrow aspirate

Volume: 2.0 ml

Minimum Volume: 1.0 ml

Container: EDTA (Lavender-top tube)

Reference Ranges: Interpretive Test (call Lab)

Test Method: Cytochemical staining of glycogen (Hotchkiss); Microscopy.

Interfering Substances: Inappropriate fixatives or anticoagulants, clotted specimen, inadequate spicules.

Revised 10/10/07 by L.. Dornak, MT (ASCP)

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