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PEROXIDASE STAIN (089-3162)

Synonyms: Myeloperoxidase Stain

CPT 4 Code(s): 88319

Test Order Mnemonic: PEROX

Applies To: Identification of acute leukemias, particularly acute myelocytic leukemia

Lab: Hematopathology

Request Form: Hematology A

Collection: Collect in EDTA. Invert specimen promptly to prevent clotting.

Storage Instructions: Deliver specimen to Hematopathology within 30 minutes of collection.

Cause For Rejection: QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangements made.

Availability: Samples are accepted from 0800 to 1400, Monday-Friday, in Room 5.142, McCullough.

Turnaround Time: 48 hours

Special Instructions: Test must be approved by Hematopathologist or Hematology/Oncology Faculty member.

Specimen: Blood, Bone marrow aspirate

Volume: 2.0 ml

Minimum Volume: 1.0 ml

Container: EDTA (Lavender-top tube)

Reference Ranges: Interpretive Test (call Lab)

Test Method: Cytochemical enzymatic reaction between peroxidase (in granules) and diaminobenzidene tetrahydrochloride (DAB).

Interfering Substances: Inappropriate fixatives or anticoagulants, clotted specimen, inadequate spicules.

Revised 10/10/07 by L.. Dornak, MT(ASCP) 

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