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PLATELET AGGREGATION: (089--0469)
Clinical Indication:

Differentiates Platelet functional status and abnormalities. based on platelet response to Arachidonic acid, ADP, Collagen, Epinephrin, and Ristocetin.
Test Mnemonic:

PLATAG
Methodology:

 Photo Optical Detection
Performed:

Must be Scheduled with Hematology Laboratory at 409-772-2293. Performed on Day Shift only, Monday through Friday.
Turnaround Time:

 24 Hours
Specimen Requirements: Patient Preparation:  Prior to drawing sample, patient must be fasting (8) hours and must not have taken aspirin or aspirin like drugs within the previous 7-10 days. (Contact lab for medication list).  Physician must provide a list of medications that have been taken

Collect:  Routine venipuncture; discard 1st mL of blood or collect other tubes (red-topped) prior to collecting sample in 4 blue-topped (3.2% sodium-citrate) tubes and one (1) purple topped tube. Immediately invert tubes gently at least 4 times to mix. Needle size should be between 19 and 21 gauge

Storage/Transport: Specimen must be delivered to the lab within one (1) hour of collection, and is stable for 3-4 hours at room temperature.  The specimen should always be handled at room temperature (20-25°C) before testing and should never be refrigerated or chilled on ice or frozen.

Stability: Stable at room temperature for 3-4 hours.

Causes for Rejection: QNS, clotted, improperly collected samples, thrombocytopenia with platelets count < 100 x 103/µl, severe hemolysis or lipemia, samples received in lab > 1 hours after  collection.
Clinical Information:

If applicable, state clinical information that is required to be provided with specimen.
Reference Range:

Final Aggregation % is reported for each agonist tested.

ADP 20 uM:                    58 – 100%       

ADP 2 uM:                       9 – 100%

Collagen 190 ug/mL:        55 – 98%

Epinephrine 10 uM:          52 – 100% 

Ristocetin 1.5 mg/mL:      55 – 99%

Ristocetin 0.5 mg/mL:     0 – 6%

Ristocetin 0.25 mglmL:      0 – 4%
CPT 4 Code:

 85576
Review: 10/18/12 by A. Huerta, BS, (MT) (ASCP), MBA

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.
 

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