SICKLE CELL SCREEN (089-0109)

Synonyms: Sickle Screen, Hgb-S Test

CPT 4 Code:  85660

Test Order Mnemonic: SICKLE

Applies To: Used to detect the presence of Hemoglobin S

Lab: Hematopathology

Request Form: Hematology A

Collection: If RBC transfusion anticipated, collect before transfusion.

Storage Instructions: Specimens not delivered to the laboratory within 2 hours should be refrigerated (stored at 2 - 8°C).

Causes for Rejection: Specimen clotted; collection in wrong tube; identification error. See special instructions.

Availability: Specimen accepted 24 hours per day at Sample Management, 5.136 McCullough.

Turnaround Time: STAT: 1 hour; Routine: 4 hours

Special Instructions: The test can be ordered only once per inpatient admission and once per month for outpatients. Additional requests require the approval of the laboratory director. Do not request on patients 0-6 months of age (Hgb-F level is too high; order Hemoglobin Electrophoresis).

Specimen: Blood

Volume: 3 ml

Minimum Volume: 1 ml

Container: EDTA (Lavender top tube); Heparin (Green top tube)

Reference Ranges: Negative: Hemoglobin-S not present. Positive: Hemoglobin-S presumed present. Follow-up with Hemoglobin Electrophoresis recommended.

Interfering substances: Elevated lipids, elevated hematocrit, elevated protein, hemolysis, extremely low hematocrit, extreme leukocytosis.

Revised 10/17/06 by L. Emanuel, MT (ASCP)