Patient should be at rest for 10 minutes prior to
Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by
collecting a discard tube prior to collecting the
blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube
should also be collected prior to collection of the blue
top to ensure sufficient sample volume. Drawing a
discard tube will displace the air from the blood
collection set tubing to ensure proper blood draw
Acceptable up to
after draw, at ROOM TEMPERATURE.
If delivery time is to be greater than 24 hours from
time of draw, double
centrifuge the specimen. Centrifuge sample for 20
minutes at 2000g.
Transfer plasma to 12x75 plastic tube. Cap tube and
re-spin for 20 minutes at 2000g.
remove from centrifuge without disturbing any platelets
and/or cell pellets that might be on the bottom or sides
of the tube.
Carefully transfer plasma into freezer tubes (plastic
screw-top cryo-tubes) for testing or freezing.
Whole Blood 24 hours at room temperature. Plasma may
be stored frozen at -20░C
for 2 weeks, or -70C for 3 years.
Causes for Rejection:
QNS, clotted, severely hemolyzed, greater than 24 hours
wrong tube (3.8% sodium citrate), high hematocrit (>
sample identification error, processed and/or stored