Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by
collecting a discard tube prior to collecting the
blue-topped (3.2% sodium-citrate) tube. For collections
with butterfly blood collection sets, a discard tube
should also be collected prior to collection of the blue
top to ensure sufficient sample volume. Drawing a
discard tube will displace the air from the blood
collection set tubing to ensure proper blood draw
Samples should be received in laboratory within ˝ hour
of draw for best results.
If delivery time is to be greater than 4 hours from time
centrifuge the specimen for 15 minutes at 2000-2500g.
Transfer plasma to 12x75 plastic tube
Cap tube and re-spin
for 15 minutes at 2000-2500g
remove from centrifuge without disturbing any platelets
and/or cell pellets that might be on the bottom or sides
of the tube.
Carefully transfer plasma into freezer tubes (plastic
screw-top cryo-tubes) for testing or freezing.
Whole blood 4 hours at room temperature. Plasma 8 hours
Frozen at -20
Causes for Rejection:
QNS, clotted, severely hemolyzed specimen, wrong tube
(3.8% sodium citrate), high hematocrit (> 55%),
sample identification error, sample
processed/transported, and/or stored improperly.