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BETHESDA [FACTOR INHIBITOR ASSAY] (089-0418) Synonyms: Bethesda Assay CPT 4 Code: 85335 Test Order Mnemonic: Bethesda Applies To: Measures levels of inhibitor of a specific factor (Factor VIII) Lab: Hematopathology Request Form: Hematology A Collection: Routine venipuncture; discard 1st mL of blood or collect other tubes (EDTA, red-topped) prior to collecting sample in blue-topped (citrate) tube. Storage Instructions: Viable for 4 hours at room temperature. If time from draw to receipt in the laboratory is to be greater than 4 hours, centrifuge the specimen; remove the plasma from red cells and snap freeze (-70°C) plasma. Snap frozen plasma is viable indefinitely. Causes for Rejection: QNS, clotted, severely hemolyzed specimen, specimen greater than 4 hours old, identification error, sample processed and/or stored improperly. Availability: Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough Bldg. Turnaround Time: Testing is performed on Day Shift only. Call lab for schedule. Special Instructions: Deliver sample to lab within ½ hour for best results. Accepted up to 4 hours from draw. Specimen: Blood Volume: 4.5 mL blood Container: 3.2% Sodium citrate tube (light Blue top tube) Reference Ranges: Normal < 0.5 units Bethesda Units Test Method: Electromagnetic Viscosity Detection Interfering Substances: Replacement or anticoagulant therapy, presence of lupus anticoagulant Reviewed 10/11/07 by W. Russo, MT(ASCP) |
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