Patient must not be on any anticoagulation therapy for 48 hours
before blood collection.
Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by collecting a
discard tube prior to collecting the blue-topped (3.2%
sodium-citrate) tube. For collections with butterfly blood
collection sets, a discard tube should also be collected prior
to collection of the blue top to ensure sufficient sample
volume. Drawing a discard tube will displace the air from the
blood collection set tubing to ensure proper blood draw volume.
Samples should be received in laboratory within ˝ hour of draw
for best results.
Acceptable up to4 hours after draw, at ROOM TEMPERATURE.
NOTE: If delivery time is to be greater than 4 hours from time
centrifuge the specimen for 15 minutes at 2000-2500g.
Separate the plasma from the cells and freeze (-200C)
Whole blood 4 hours at room temperature. Plasma 4 hours at
20+/- 5°C. Frozen 15 days at -20°C, or 1 month at -80C.
Causes for Rejection:
clotted, severely hemolyzed specimen, specimen greater than 4
hours old, wrong tube (3.8% sodium citrate), high hematocrit (>
55%), identification error, sample processed/transported, and/or