Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by collecting a
discard tube prior to collecting the blue-topped (3.2%
sodium-citrate) tube. For collections with butterfly blood
collection sets, a discard tube should also be collected prior
to collection of the blue top to ensure sufficient sample
volume. Drawing a discard tube will displace the air from the
blood collection set tubing to ensure proper blood draw volume.
Centrifuge the specimen for 15 minutes at 2000-2500g.
Separate the plasma from the cells and freeze at (-200C).
Whole blood 4 hours at room temperature.
storage: 8 hrs at 20 +/- 50C. 1 month at -200C.
Causes for Rejection:
clotted, severely hemolyzed specimen, specimen greater than 4
hours old, wrong tube (3.8% sodium citrate), high hematocrit (>
55%), sample identification error, sample processed/transported,
and/or stored improperly.