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HEMOSIDERIN STAIN-BONE MARROW ASPIRATE (089-8313), Synonyms: Prussian Blue Iron Stain, Iron Stain CPT 4 Code: Bone Marrow Aspirate 88313; Peripheral blood/body fluids 85536 Test Order Mnemonic: IRONBM (Bone Marrow); Iron Stain (Peripheral fluids) Applies To: Classification of anemias, detection of CNS hemorrhage and to evaluate bone marrow iron stores. Lab: Hematopathology Request Form: Hematology A Collection: Collect in EDTA. Invert promptly to prevent clotting. Storage Instructions: All types of specimens should be delivered to lab promptly. Bone marrow aspirates should be delivered within 30 minutes. Cause For Rejection: QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangements made. Availability: Bone Marrow Aspirate samples are accepted 8AM - 2PM, Monday-Friday, Room 5.142, McCullough. Body fluids and peripheral blood accepted 24 hours. Turnaround Time: 24 hours Specimen: Bone marrow aspirate, CSF, serous fluids, blood Volume: 1.0 ml Minimum Volume: 0.5 ml Container: EDTA (Lavender-top tube); CSF- standard sterile tube Reference Range: Normal bone marrow is positive for hemosiderin. All body fluids should be negative. Test Method: Cytochemical demonstration of iron-bearing pigment (Perl’s Prussian Blue); Microscopy. Interfering Substances: Inappropriate fixatives or anticoagulants, clotted specimen, inadequate spicules. Revised 10/10/07 by L. Dornak, MT (ASCP) |
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