Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by collecting a
discard tube prior to collecting the blue-topped (3.2%
sodium-citrate) tube. For collections with butterfly blood
collection sets, a discard tube should also be collected prior
to collection of the blue top to ensure sufficient sample
volume. Drawing a discard tube will displace the air from the
blood collection set tubing to ensure proper blood draw volume.
Centrifuge the sample 15 minutes at 2500g.
Separate the plasma from the cells and transport at 20 +/- 5
Whole blood 4 hours at room temperature.
Plasma storage 8 hours at
Causes for Rejection:
clotted, severely hemolyzed specimen, plasma specimen greater
than 8 hours old, wrong tube (3.8% sodium citrate), high
hematocrit (> 55%), sample identification error, sample
processed/transported, and/or stored improperly.