Laboratory Test Directory

Reptilase Time: (089-0514)

Clinical Indication:

 Useful in differentiating causes of prolonged thrombin times.

Test Mnemonic:

 REPTILASE

Methodology:

 Electromagnetic Viscosity Detection

Performed:

 Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough Bldg.

Turnaround Time:

  STAT: 1 hour; ROUTINE: 4 hours

Specimen Requirements:

Collect:  Blue-topped (3.2% sodium-citrate) tube. Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume.

Specimen Preparation: Centrifuge the sample 15 minutes at 2500g. 

Storage/Transport:NOTE: Separate the plasma from the cells and transport at 20 +/- 5 ⁰C

Stability: Whole blood 4 hours at room temperature. Plasma storage 8 hours at 20+/- 5°C.   

Causes for Rejection: QNS, clotted, severely hemolyzed specimen, plasma specimen greater than 8 hours old, wrong tube (3.8% sodium citrate), high hematocrit (> 55%), sample identification error, sample processed/transported, and/or stored improperly.

Clinical Information:

Interfering Substances: none

Reference Range:

13.6 - 19.2  seconds

CPT 4 Code:

 85635

Reviewed: 3/20/13

W.Russo, MT(ASCP)

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.