Blue-topped (3.2% sodium-citrate) tube.
Routine venipuncture; discard 1st mL of blood by collecting a
discard tube prior to collecting the blue-topped (3.2%
sodium-citrate) tube. For collections with butterfly blood
collection sets, a discard tube should also be collected prior
to collection of the blue top to ensure sufficient sample
volume. Drawing a discard tube will displace the air from the
blood collection set tubing to ensure proper blood draw volume.
after collection, at
delivery time is to be greater than 4 hours from time of draw, centrifuge
the sample for 10 minutes at 2500g.
Separate the plasma from the cells and store at room
patients on heparin therapy, the blood must be centrifuged and
the plasma separated from cells within 1 hour of collection.
Whole blood 4 hours at room temperature.
Plasma storage 8 hours at
Patients on heparin therapy, plasma is stable for 2 hours at
Causes for Rejection:
clotted, severely hemolyzed specimen, specimen greater than 4
hours old or 2 hours old for patients on heparin therapy, wrong
tube (3.8% sodium citrate), high hematocrit (> 55%),
sample identification error, sample processed/transported,
and/or stored improperly.