ACID FAST CULTURE, non-sterile sites (088-0005)
Synonyms: AFB Culture, non-sterile sites; TB Culture, non-sterile sites
CPT 4 Code: 87116
Test Order Mnemonic: C ACON
Test Includes: Acid fast smear, isolation and identification of Mycobacterium species and antimicrobial susceptibility testing when appropriate
Applies To: Mycobacterium species
Request Form: Microbiology
Collection: Collect specimens in a sterile, leak-proof container. For sputum collection, use the sputum collection kit. Collection complete first morning urine for AFB culture.
Storage Instructions: Refrigerate
Causes for Rejection: Sputum from patient not in "airborne precautions", more than three samples per week submitted on the same patient (respiratory sources only), feces on patients other than HIV+, leaking container, insufficient quantity, samples submitted on a swab, more than two feces per week.
Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough.
Turnaround Time: Smears: 1 day; cultures are held 6 weeks
Special Instructions: Collect sputum specimens in sputum collection kits available through Materials Management.
Specimen: Sputum, respiratory sources, gastric aspirates, feces, autopsy sources, abscesses, skin lesions, urine samples
Minimum Volume: 3 mL
Container: Sterile container, sputum collection kit
Critical Values: Positive smear, first occurrence; Mycobacterium tuberculosis on culture
Revised 6/22/2011 by T. Friloux