Synonyms: AFB Culture, sterile site
CPT 4 Code: 87116
Test Order Mnemonic: C AFB
Test Includes: Acid fast smear (except for bone marrow), isolation, and identification of Mycobacterium species and antimicrobial susceptibility testing when appropriate.
Applies To: Mycobacterium species
Request Form: Microbiology
Collection: Aseptic technique
Storage Instructions: Refrigerate all specimens EXCEPT bone marrow and CSF. Bone marrow and CSF are stored at room temperature.
Causes for Rejection: Clotted specimens, swabs
Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough.
Turnaround Time: Smears: 1 day; cultures are held 6 weeks
Specimen: Bone marrow, bone marrow core, CSF, tissues, and body fluids
Minimum Volume: 0.5 ml, 3 ml minimum for CSF specimens
Container: Bone marrow aspirate: heparin-tube or Pedi-Isolator tube; Other sources: sterile container or test tube
Critical Values: Smear positive for acid fast bacilli, first occurrence; culture positive for Mycobacterium tuberculosis
Test Method: Culture - agar and broth
Revised 6/22/2011 by T. Friloux